一、Vaccine Production Process and Optimization

　　1、Large-scale fermentation of human vaccines and cell culture technology and process

　　　　⊙Large-scale fermentation of escherichia coli

　　　　⊙Large-scale fermentation of yeast

　　　　⊙Large-scale culture of animal cells

　　2、People downstream separation and purification technologies and processes vaccine

　　　　⊙Separation and purification of viral vaccine

　　　　⊙Particle separation and purification of recombinant VLP

　　　　⊙And the separation and purification of polysaccharide conjugate vaccine

　　3、Veterinary vaccines large-scale fermentation and cell culture technology and process

　　　　⊙E. coli large-scale fermentation

　　　　⊙Large-scale fermentation yeast

　　　　⊙Large-scale animal cell culture

　　　　⊙Large-scale culture of baculovirus

　　4、Veterinary vaccines downstream separation and purification technologies and processes

　　　　⊙Separation and purification of viral vaccine

　　　　⊙Particle separation and purification of recombinant VLP

　　二、Large-scale production of recombinant protein technology and process development

　　1、Recombinant E. coli fermentation technology and process

　　2、Recombinant yeast fermentation technology and process

　　3、Recombinant animal cell culture technology and process

　　4、E. coli expression of recombinant protein purification technology and process

　　　　⊙Inclusion body refolding and purification technologies

　　　　⊙Soluble expression and purification technology

　　5、Yeast expression of recombinant protein purification technology and process

　　　　⊙Secretory expression and purification technology

　　　　⊙Lyse the cells express intracellular purification technology

　　6、Animal cell expression of recombinant protein purification technology and process